Application

Immunoblotting: Recommended antibody dilution 1:1500-3000

Immunoprecipitation: Recommended antibody dilution 1:50

Immunohistochemistry: Recommended antibody dilution 1:600-1500

Optimal antibody dilutions must be determined by end-user.

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2 is an antibody against Bcl-2 for use in WB, IP, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

In C. elegans, ced-3 and ced-4 were identified as genes essential for programmed cell death, while ced-9 was found to be a regulator of cell death by preventing apoptosis. The first mammalian homolog for ced-3 was described in 1988 as Bcl-2 which is involved in B-cell lymphomas (Vaux and Adams, 1988, Nature 335: 440). Bcl-2 transfected B cells were shown to be resistant towards apoptosis normally induced in B cells by IL-3 withdrawal: for the first time it was shown that the pathway toward tumorigenesis depends not only on the ability to escape growth control but also depends on the ability to prevent apoptosis. The bcl-2 gene codes for a 25 kDa protein. The C terminal 21 amino acids encode a stretch of hydrophobic amino acids that are important in membrane docking: Bcl-2 resides on the cytoplasmic face of the mitochondrial outer membrane, the nuclear envelop, and the endoplasmic reticulum. Deletion of the C terminus does not abrogate Bcl-2 survival function! Most Bcl-2 homologs have this hydrophobic C terminal domain, though they not necessarily are located on membranes but are cytosolic (e.g. Bax).

{http://www.celldeath.de/encyclo/}

Immunogen

Peptide against mouse Bcl-2 a.a. 68-86 RTSPLRPLVATAGPALSPV

Epitope: a.a. 68-86 of mBCL2

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-436

Physical form

Whole serum without preservatives

Specificity

AB1722 recognizes Bcl-2 alpha and beta isoforms in mouse and rat. Cross reactivity with other species is unknown

Storage and Stability

Store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze thaws.